Univariate and multivariate trend analyses of all markers combined. (A). Results of the univariate and multivariate trend analyses of combined markers H3K4me3, H3K9me3, and H4K20me3. HR represents the hazard ratio for each unit of increase, thus each additional marker showing unfavorable expression. 95% CI: 95% confidence interval for each HR. OS: overall survival; DSS: disease-specific survival; LRRFS: locoregional recurrence-free survival; DRFS: distant recurrence-free survival. (B). Kaplan-Meier curves are shown for OS, including the number of patients in each patient group (n), based on the number of markers showing unfavorable expression. Patients were divided into the following groups: all favorable (group 1; H3K4me3 low and both H3K9me3 and H4K20me3 high), one out of three unfavorable (group 2), two out of three unfavorable (group 3), and all unfavorable (group 4; H3K4me3 high and both H3K9me3 and H4K20me3 low). Cumulative incidence curves, accounting for competing risks, are shown for DSS, LRRFS and DRFS. Multivariate p-values have been included in each of the combined marker graphs.