Figure 4

DIM ligand-independent activation of ERα is mediated by the PKA signaling pathway. CYP1A1 mRNA level (A) and GREB1 mRNA level (B) in MCF7 cells grown in estrogen-free media for three days and then treated or not with 50 μM ICI or 10 μM H89 for 24 h prior to the addition of DMSO, 100nM E2, 10 μM DIM, 10 μM DIM + 50 μM ICI or 10 μM DIM + 10 μM H89 for 24 h. Results are shown as percent of maximum induction and represent the mean of three independent experiments with standard deviation.