Figure 2

Typical expression pattern of the macrophage markers CD68, CD11c, CD163 and MRC1 in a lymph node. Exemplary fields of view (original magnification 40x) showing the typical expression pattern of the stained macrophage markers CD68, CD11c, CD163 and MRC1 in lymph node specimens. a) CD68: The marker shows a cytoplasmatic staining. The shape of the stained cells includes predominantly round cells, but some cells also show a spindle-shape. CD68 is expressed in the follicles, in the interfollicular zone and in the sinus. Besides a distinct staining of the macrophages, in lymph node metastasis specimens a pale staining of tumor cells was also detectable. b) CD11c: The marker shows a cytoplasmatic expression pattern with an accentuation of the plasma membrane. The shape of the stained cells includes predominantly round cells. CD11c expressing cells were mainly found in the follicles and in the interfollicular zone. c) CD163: The marker shows a cytoplasmatic expression pattern with an accentuation of the plasma membrane. The shape of the stained cells includes predominantly spindle-shaped cells. CD163 expression can predominantly be found in the lymph node sinus. The follicles are largely missing CD163 expressing cells. d) MRC1: The marker shows a cytoplasmatic expression pattern with an accentuation of the plasma membrane. The shape of the stained cells includes predominantly spindle-shaped cells. MRC1 expression can predominantly be found in the lymph node sinus. The follicles are largely missing MRC1 expressing cells.