ANXA1 expression inversely significantly correlated with COX-2 expression. (A) CCK8 assay showed that ANXA1 down-regulation leaded to increased cell viability of N87. The results are expressed as the ratio of viable cells at 48 h compared to that at 0 h. *P < 0.05 as compared to the control. (B) Western blot analysis showed ANXA1 and COX-2 expression in N87 cancer cells with ANXA1 down-regulation. (C) Upper: CCK8 assay showed that overexpression both ANXA1 and COX-2 simultaneously abolish the effect of inhibition of cell proliferation by ANXA1 alone in AGS cells. Lower: Western blot analysis showed ANXA1 and COX-2 expression in AGS cancer cells with ANXA1 overexpression alone or both ANXA1 and COX-2 overexpression. (D) Graphical representation of the intensity of ANXA1 and COX-2 staining in gastric cancer and non-cancerous tissues. N, non-cancerous tissues; T: primary tumor tissues. T tests were used to analyze the difference of the proliferation rate from each group or the intensity of ANXA1 and COX-2 protein levels between gastric cancer and non-cancerous tissues. *P < 0.05 as compared to the control. (E) Representative pictures showing expression patterns of ANXA1 and COX-2 in human gastric cancers from the same patients. Original magnification of the big pictures: 100×; Original magnification of the small pictures: 400×. (F) Graphical representation of levels of ANXA1 and COX-2 immunostaining in human gastric cancers from the same patients.