ANXA1 regulates cell viability and invasion of gastric cancer cells. (A, B) Immunofluorescence analysis (upper panel) and western blot analysis (lower panel) showed ANXA1 expression from AGS (left) and N87 (right) cancer cells that were transfected with pcDNA3.1-ANXA1 expression vectors. (C-H) Gastric cancer cells (AGS and N87) were transfected with pcDNA3.1 vector or pcDNA3.1-ANXA1 expression vectors and cell proliferation (C, D), colony formation (E, F) and invasion ability (G, H) were performed. The histogram shows the percentage of cells that formed colony or penetrated the membrane. Two-way ANOVA was performed to analyze the overall difference of the proliferation rate between cells transfected with pcDNA3.1 vector or pcDNA3.1-ANXA1. *P < 0.05 as compared to the control. Unpaired t test was carried out to analyze the difference of the ability of colony and invasion between the two groups. *P < 0.05 as compared to the control.