Expression of ANXA1 in human gastric cancer cells. (A, B) Levels of ANXA1 mRNA (A) and protein (B) in 7 gastric cancer cell lines and 1 immortalizated gastric cell, GES1; ANXA1: 37kd, ß-actin: 42kd. (C) Localization and intensity of ANXA1 in AGS and N87 cell lines determined by immunofluorescence analysis; (D) Proliferative rates of AGS and N87 determined by CCK8 assay; Two-way ANOVA was performed to analyze the overall difference of the proliferation rate between two cell types. ***P < 0.001. (E) Differences of invasion ability of AGS and N87 shown by two-chamber invasion assay. Unpaired t test was carried out to analyze the difference of the invasion cell numbers between the two cell types. ***P <0.001.