Expression profiles of ANXA1 in human gastrointestinal carcinomas and noncancerous tissues by Real-time PCR and Western blot analysis. (A) Normalized mRNA values of ANXA1 in human gastrointestinal tissues. Bile duct tissue with the lowest expression of ANXA1 mRNA was designated as the calibrator and the normalized value for each tissue was divided by this calibrator. (B) Differential expression of ANXA1 mRNA in 6 different tumor types. The fold changes (y axis) are calculated by the ratio of the relative amounts of mRNA in the tumor vs healthy tissue in log scale. (C) Differential expression of ANXA1 protein in the six tumor types determined by western blotting assay. N, matched noncancerous tissue; T, tumor. ANXA1: 37kd, ß-actin: 42kd. (D) Graphical representation of the ratio of T/N in these six types of tumor from (C). N, matched noncancerous tissue; T, tumor. CHO, cholangiocarcinoma; PDC, pancreatic carcinoma; ESC, esophageal carcinoma; GC, gastric carcinoma; CRC, colorectal carcinoma; HCC, hepatocellular carcinoma.