Figure 2

miRNAs significantly different in triple negative breast cancer with lymph node metastases. Supervised hierarchical cluster analysis was performed on miRNAs significantly altered between tumour (red branches, top) and normal adjacent tissue samples (blue branches, top) in A) lymph node negative and B) lymph node positive patients. Similarity in the expression patterns between miRNAs (branches shown on the left-hand panel) and between samples (branches shown on the top panel) was measured using Pearsons correlation. Distances between clustered branches represent the average distances between miRNAs and samples in the cluster. The height of each branch represents the degree of similarity within the cluster. miRNAs are coloured according to their expression level, where up-regulated expression is represented by red, down-regulated expression is represented by blue, and equal expression is represented by yellow. C) Venn diagram representing the overlap between miRNAs regulated in lymph node negative patients (37 miRNAs, left-hand circle), lymph node positive patients (46 miRNAs, right-hand circle) and in the lymph node metastases of lymph node positive patients (63 miRNAs, bottom circle). D) Histogram depicting the fold regulation of the 27 miRNAs (highlighted in red in C) in tumour compared to NAT in lymph node negative tumours (white), lymph node positive tumours (black) and lymph node metastases (grey). Two-fold regulation is depicted by the red line.