PQ1’s effects on GJIC in the presence of kinase inhibitors. Eight hundred thousand cells were seeded into six-well plates. A) SW480 cells were treated with no treatment (control) and DMSO (control) for 1 hour. B) SW480 cells were pre-treated with kinase inhibitors (Calphostin C or Staurosporin) for 1 hour. After pre-treatment, cells were treated with 200 nM PQ1 for 1 hour. SL/DT was preformed after 1 hour of PQ1 treatment. Cells with Lucifer yellow indicate in white and the point of entry for Lucifer yellow indicates in red line. These images represent one set of three independent experiments.