PQ1 leads to p44/42 MAPK expression. Cells were treated with: no treatment (control), DMSO (control) and 200 nM PQ1 for 1 hour. Levels of phospho-p44/42 MAPK (active p44/42 MAPK) expression was examined by western blot analysis. GAPDH was used as a loading control. A) Level of active p44/42 MAPK was detected using anti-phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody specific for endogenous active p44/42 MAPK. B) Graphical presentation of three independent experiments showing pixel intensities of active p44/42 MAPK normalized to control. *P value is <0.05 compared to control. IB = Immunoblot against phosphor-p44/42 MAPK.