Figure 3

PQ1 causes an increase in gap junction activity of SW480 cells. Eight hundred thousand cells were seeded into six-well plates. SL/DT assay was performed after the following conditions: A) SW480 cells were treated with no treatment (control) and DMSO (control) for 1 hour; B) SW480 cells were treated with 50 nM PQ1, 200 nM PQ1 and 500 nM PQ1 for 1 hour; C) SW480 cells were treated with 200 nM TPA and 200 nM TPA + 200 nM PQ1 for 1 hour; and D) SW480 cells were treated with 100 μM CBX and 100 μM CBX + 200 nM PQ1 for 1 hour. Cells with Lucifer yellow indicated in white and the point of entry for Lucifer yellow indicated by red line. These images represent one set of three independent experiments.