Overexpression of Cx43 causes a decrease in viability of SW480 cells. Eight hundred thousand cells were seeded into six-well plates. Cells were transfected with optifect for 24 hours. Transfection conditions were: Control, Empty Vector, and Cx43 vector. After 24 hours, viability and proliferation assays were performed. A) Proliferation of SW480 cells. B) Viability of SW480 cells. Data were obtained in three independent experiments and are represented as the mean ± SD.