Figure 3

Omeprazole inhibits migration and invasion in vitro and metastasis in vivo . (a) Migration. MDA-MB-231 cells were treated with DMSO, TCDD or omeprazole, and cell migration was determined using a scratch assay as outlined in the Methods. (b) Decreased MMP-9 and CXCR4 expression. MDA-MB-231 cells were treated with DMSO, TCDD or omeprazole, and MMP-9 and CXCR4 mRNA levels were determined by real time PCR as outlined in the Methods. Results are means ± SE for at least 3 replicate determinations and significant (p < 0.05) inhibition is indicated. H&E staining (c) and immunostaining (d) of lung tissue from in vivo studies. Lung tissue sections from animals treated with corn oil (control) or omeprazole (100 mg/kg/d) were obtained for H&E and immunostaining as outlined in the Methods. Image magnification 40×; inset magnification 100 × .