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Figure 2 | BMC Cancer

Figure 2

From: The aryl hydrocarbon receptor ligand omeprazole inhibits breast cancer cell invasion and metastasis

Figure 2

Role of the AHR in mediating inhibition of MDA-MB-231 cell invasion by omeprazole. (a) AHR silencing. MDA-MB-231 cells were transfected with siCtl (control) and siAHR (targeting AHR) oligonucleotides treated with DMSO, TCDD or omeprazole, and effects on cell invasion were determined in a Boyden Chamber assay as outlined in the Methods. Significant (p < 0.05) inhibition of invasion (*) and reversal of these effects by siAHR (**) are indicated. Similar results were observed with another siAHR oligonucleotide (data not shown). AHR antagonists 3′,4′-dimethoxy-α-naphthoflavone (b) and 3′-methoxy-4′-nitroflavone (c) block omeprazole-induced effects on invasion. Cells were treated with DMSO, omeprazole and the AHR antagonists alone or in combination, and cell invasion was determined in a Boyden chamber assay as outlined in the Methods. Significant (p < 0.05) inhibition of invasion (*) and rescue by the AHR antagonists (**) is indicated. (d) AHR antagonist and silencing inhibits induction of CYP1A1 mRNA by omeprazole. Cells were treated with DMSO, omeprazole and the antagonists alone or in combination (right and middle panel) or transfected with siCtl or siAHR and treated with DMSO, TCDD or omeprazole, and CYP1A1 mRNA levels were determined by real time PCR as outlined in the Methods. Significant (p < 0.05) induction of CYP1A1 (*) and reversal of this effect (**) are indicated. Results (a - d) are expressed as means ± SE for at least 3 replicate experiments for each treatment group.

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