Figure 2
Checkpoint activation and DNA damage protein biomarker responses in HT29 cells following treatment with cytotoxic chemotherapeutic agents. HT29 cells were treated with approximately 5-times the single agent GI50 of gemcitabine (Gem, 0.2 μM), camptothecin (CPT, 1 μM), cisplatin (CP, 125 μM), oxaliplatin (OxPt, 250 μM), doxorubicin (Dox, 3 μM) or etoposide (Etop, 50 μM) for 24 hours. Changes in protein expression levels were determined by immunoblotting.