Figure 3
Adoptive immunotherapy produced a tumor-specific immune response and prolonged survival rate of LL/2 tumor-bearing mice. Splenic lymphocytes of all groups were isolated after the third immunization and injected i.v (1 × 107cells per mouse) into mice which were inoculated LL/2 tumor cells (1 × 106 cells per mouse) subcutaneously 3 days ago. Adoptive immunotherapy of splenic lymphocytes was repeated every 2 days for 5 times. (A) The schedule of prophylactic immunotherapy. (B) Tumor volume of mice adopted with lymphocytes from co-expression IL-18 and GM-CSF group co-expression IL-18 and GM-CSF group effectively inhibited compared with other groups (p < 0.01, n = 7). (C) Kaplan-Meier survival analysis showed that the survival of co-expression IL-18 and GM-CSF group was significantly prolonged (p < 0.01, n = 7). Error bars represent SD.