Figure 3

The RhoC-mediated effects of TGF-β1 and VEGF on EMT and lamellipodia formation of ovarian carcinoma cells. Morphologically, the treatment of VEGFR and TGF-β1R inhibitors might result in the increased ratio of round CAOV3 and transfectant cells, but the exposure to both growth factors could make more OVCAR3 and transfectant cells become spindle (A). Both inhibitors could decrease the ability of CAOV3 cells or their transfectants to form lamellipodia by F-actin staining, while RhoC overexpresion could enhance the effect. Growth factors induced the OVCAR3 and RhoC siRNA transfectants to form lamellipodia, while RhoC knockdown caused the weaker ability of both cells (B).