Figure 1

The involvement of RhoC in EMT of ovarian carcinoma cells. The mRNA and protein expression of RhoC was screened in ovarian carcinoma cells (SKOV3, OVCAR3, CAOV3, HO8910 and ES-2) by real-time PCR (A) and Western blot (B). CAOV3 cells were transfected with RhoC-expressing plasmid and confirmed by real-time PCR and Western blot (C). After transfection of RhoC siRNA, RhoC expression became weaker in OVCAR3 by real-time PCR and Western blot (D). CAOV3 cells became spindle after ectopic RhoC expression, while RhoC knockdown caused OVCAR3 morphorlogically round (E). There was a down-regulated expression of E-cadherin mRNA, and up-regulated expression of N-cadherin and a-SMA mRNA in CAOV3 transfectants by real-time PCR (F). After the treatment of RhoC siRNA, there was an increased expression of E-cadherin mRNA in OVCAR3 cells by real-time PCR, while the converse was true for the expression of N-cadherin and a-SMA mRNA (F). * compared with control and mock, p < 0.05.