Figure 3

Left: AXIN2 mRNA expression level of samples selected from cluster 2A, 2B1 and 2B2. Expression levels were analyzed by Q-PCR using ACTB housekeeping gene for normalization. Thick horizontal lines within the boxes indicate the median value. Thin dashed horizontal lines indicate the mean value. Middle: bisulfite sequencing results of the 0.5Kb upstream region of the first exon of AXIN2. CpG sites are represented by a white or a black circles for unmethylated or methylated sites, respectively. Right: AXIN2 mRNA expression level in colorectal cancer cell lines HCT116 (MSI, white bar) and Caco2 (MSS, stripped bar), and tumors, both sessile serrated adenomas (black bars) and MSI carcinomas (white bars), ordered from lower to higher (top to bottom). Arrows between middle and right panel connect those cases for which both the bisulfite sequencing and the mRNA expression results are shown in the figure.