Table 1 List of primary antibodies used for IHC, ICC, and WB analysis
Primary antibody | Antigen retrieval for IHC | Dilution & incubation time | Product number | Vendor | ||
|---|---|---|---|---|---|---|
IHC | ICC | WB | ||||
Anti-UPIa | none | 1:100 o.n., 4°C | 1:50 1 hour, r.t. | 1:1000 o.n., 4°C | sc-15173 (C-18) | Santa Cruz Biotechnology, Santa Cruz, CA |
Anti-Cytokeratin | Proteinase- K | 1:800 1 hour, r.t. | 1:50 1 hour, r.t. | 1:1000 o.n., 4°C | M3515 (AE1/AE3) | Dako, Carpinteria, CA |
Anti-EGFR | Sodium Citrate | 1:100 o.n., 4°C | 1:25 1 hour, r.t. | 1:1000 o.n., 4°C | sc-03 (1005) | Cell Signaling Technology, Boston, MA |
Anti-COX-2 | Sodium Citrate | 1:500 o.n., 4°C | 1:50 1 hour, r.t. | 1:1000 o.n., 4°C | 160126 | Cayman Chemical, Ann Arbor, MI |
Anti-p65 | none | 1:500 1 hour, r.t. | - | 1:1000 o.n., 4°C | 610868 | BD Transduction Laboratories, San Jose, CA |
Anti-Vimentin | - | - | 1:100 1Â hour, r.t. | - | sc-7557 (C-20) | Santa Cruz Biotechnology, Santa Cruz, CA |
Anti-Ki67 | - | - | 1:50 1Â hour, r.t. | - | M7240 (MIB-1) | Dako, Carpinteria, CA |
Anti-PDGFR | - | - | 1:25 1 hour, r.t. | 1:1000 o.n., 4°C | SC-338 (C-20) | Santa Cruz Biotechnology, Santa Cruz, CA |
Anti-VEGFR | - | - | 1:25 1 hour, r.t. | 1:500 o.n., 4°C | 2467 (55B11) | Cell Signaling Technology, Boston, MA |
Anti-phospho-ERK1/2 | - | - | - | 1:1000 o.n., 4°C | sc-7383 (E-4) | Santa Cruz Biotechnology, Santa Cruz, CA |
Anti-p27 | - | - | - | 1:1000 o.n., 4°C | sc-528 (C-19) | Santa Cruz Biotechnology, Santa Cruz, CA |