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Table 1 List of primary antibodies used for IHC, ICC, and WB analysis

From: Animal model of naturally occurring bladder cancer: Characterization of four new canine transitional cell carcinoma cell lines

Primary antibody Antigen retrieval for IHC Dilution & incubation time Product number Vendor
IHC ICC WB
Anti-UPIa none 1:100 o.n., 4°C 1:50 1 hour, r.t. 1:1000 o.n., 4°C sc-15173 (C-18) Santa Cruz Biotechnology, Santa Cruz, CA
Anti-Cytokeratin Proteinase- K 1:800 1 hour, r.t. 1:50 1 hour, r.t. 1:1000 o.n., 4°C M3515 (AE1/AE3) Dako, Carpinteria, CA
Anti-EGFR Sodium Citrate 1:100 o.n., 4°C 1:25 1 hour, r.t. 1:1000 o.n., 4°C sc-03 (1005) Cell Signaling Technology, Boston, MA
Anti-COX-2 Sodium Citrate 1:500 o.n., 4°C 1:50 1 hour, r.t. 1:1000 o.n., 4°C 160126 Cayman Chemical, Ann Arbor, MI
Anti-p65 none 1:500 1 hour, r.t. - 1:1000 o.n., 4°C 610868 BD Transduction Laboratories, San Jose, CA
Anti-Vimentin - - 1:100 1 hour, r.t. - sc-7557 (C-20) Santa Cruz Biotechnology, Santa Cruz, CA
Anti-Ki67 - - 1:50 1 hour, r.t. - M7240 (MIB-1) Dako, Carpinteria, CA
Anti-PDGFR - - 1:25 1 hour, r.t. 1:1000 o.n., 4°C SC-338 (C-20) Santa Cruz Biotechnology, Santa Cruz, CA
Anti-VEGFR - - 1:25 1 hour, r.t. 1:500 o.n., 4°C 2467 (55B11) Cell Signaling Technology, Boston, MA
Anti-phospho-ERK1/2 - - - 1:1000 o.n., 4°C sc-7383 (E-4) Santa Cruz Biotechnology, Santa Cruz, CA
Anti-p27 - - - 1:1000 o.n., 4°C sc-528 (C-19) Santa Cruz Biotechnology, Santa Cruz, CA
  1. Notes: o.n. - over night; r.t. - room temperature.