Tumorigenic behavior of K9TCC cells. (A) The primary K9TCC cell were subcutaneously inoculated in athymic mice (n = 5/cell line, 1.5 × 106 cells/mouse with 1:1 Matrigel/PBS) to confirm tumorigenic behavior of tested K9TCC cells. Human UMUC-3 cells were used as positive controls (3 × 106 cells/mouse with 1:1 Matrigel/PBS). Tumor length was measured after three weeks. Values were represented as the mean +/-S.E. of tumors length (n = 5 mice). (B) Representative images of H&E and cytokeratin expression (brown color) of K9TCCs and UMUC-3 xenograft tumors. Objectives 20× with scale bar 50 μm.