Characterization of primary K9TCC cells
. The expression of UPIa was used to confirm the urothelium cell-origin of established K9TCC cells K9TCC#1Lillie, K9TCC#2Dakota, K9TCC#4Molly, and K9TCC#5Lilly (brown color) in vitro using ICC. Cytokeratin was expressed in the membrane in all tested K9TCC cells and confirmed the epithelial-cell origin of established K9TCC cells. Weak expression of vimentin was observed only in K9TCC#1Lillie and K9TCC#2Dakota cells. Ki67 expression was positive in nucleus, confirming that K9TCC cells were undergoing cell-cycle division. All tested K9TCC cells showed strong COX-2 and PDGFR expressions. Moderate expressions of EGFR and low expressions of VEGFR were detected in all tested K9TCC cells. Objective 20× with scale bar 50 μm.