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Figure 6 | BMC Cancer

Figure 6

From: FHL1C induces apoptosis in notch1-dependent T-ALL cells through an interaction with RBP-J

Figure 6

Overexpression of FHL1C induced apoptosis of Jurkat cells involving multiple effectors and pathways. (A) Jurkat cells were transfected with pEGFP or pEGFP-FHL1C by using the Nucleofection method. The cells were harvested 48 h post-transfection, and the mRNA levels of Hes1, Hes5 and c-Myc were detected by real time RT-PCR, with β-actin as a reference. (B) Jurkat cells were transfected as in (A). The protein level of c-Myc was determined by using Western blotting. (C,D) Cell lysates were prepared from Jurkat cells transfected with pEGFP or pEGFP-FHL1C for 48 h. AKT and phosphorylated AKT (pAKT) were analyzed by Western blotting (C). The relative levels of AKT and pAKT were quantified and compared, with β-actin as an internal control (D). (E-G) Jurkat cells were transfected with pEGFP or pEGFP-FHL1C by using the Nucleofection method. Cells were harvested 24 h post-transfection, and the cytosolic and nuclear extracts were fractioned. P50, c-Rel and IκB were determined by Western blotting (E). The relative levels of P50 (F) and c-Rel (G) were quantified and compared, with β-actin as an internal control. Bars = means ± S.D (n = 3), *P < 0.05, **P < 0.01.

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