FHL1C induced apoptosis of Jurkat cells through repressing RBP-J. (A) Jurkat cells were transiently transfected with pEGFP, pCMX-VP16-RBP-J, pEGFP-FHL1C or pEGFP-FHL1C plus pCMX-VP16-RBP-J. The percentage of apoptotic (Annexin V+) cells in EGFP+ cell population was measured 24 h after transfection. (B) Constitutively active RBP-J blocked FHL1C-induced apoptosis in Jurkat cells. Jurkat cells were transiently transfected with 1 μg of pEGFP-FHL1C alone or in combination with increasing amounts (0.2, 0.5, 1.0 μg) of pCMX-VP16-RBP-J. Cell apoptosis was measured by Annexin V staining on different days after transfection. The percentages of apoptotic (Annexin V+) cells in EGFP+ cell population were shown. Bars = means ± S.D (n = 3), *P < 0.05, **P < 0.01.