Overexpression of FHL1C in Jurkat cells resulted in reduced cell viability. (A) Jurkat cells (5 × 106) were transfected with pEGFP or pEGFP-FHL1C by using the Nucleofection method. The numbers of viable EGFP-positive cells were determined every 12 h by cell counting and FACS analysis. (B, C) Cell cycle progression of Jurkat cells in (A) was determined 48 h post-transfection by FACS after PI staining (B). Cells in each phase were compared between the two groups (C). (D) Total viability of Jurkat cells transfected in (A) was monitored by using trypan blue exclusion assay. Bars = means ± S.D (n = 3), *P < 0.05, **P < 0.01.