Skip to main content
Figure 1 | BMC Cancer

Figure 1

From: Single cell mutational analysis of PIK3CA in circulating tumor cells and metastases in breast cancer reveals heterogeneity, discordance, and mutation persistence in cultured disseminated tumor cells from bone marrow

Figure 1

Single cell isolation and identification. Individual tumor cells (TCs) from primary or metastatic tissue, blood, or bone marrow were isolated by the MagSweeper and immunostained (200×). Panel (A) shows examples of CTCs and DTCs (CK+, CD45-, DAPI+); small round circles in two frames of (A) are autofluorescing residual magnetic microbeads; Panel (B) shows an example of TCs from tumor tissue (CK+, CD45-, DAPI+); Panel (C) shows examples of WBCs (CK-/very weak, CD45+, DAPI+). Green = cytokeratin; red = CD45; blue = DAPI nuclear stain.

Back to article page

BMC Cancer

ISSN: 1471-2407

Contact us