Wound-healing migration assay of CNF1-treated Gl261 cells. (A) Representative images of wound in untreated cells (left column) and CNF1-treated cells (right column) at different time points (0, 8, 24 and 48 hours). The data demonstrate the inability of CNF1-treated cells to invade the wound area. (B) Quantitative plots of the wound size at different time points in the two conditions (CNF1 and control). Data shown are representative of three independent experiments. All data represent means ± SEM. (two-way ANOVA, **p < 0.001). Scale bar = 100 μm.