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Figure 2 | BMC Cancer

Figure 2

From: MiR-205 inhibits cell apoptosis by targeting phosphatase and tensin homolog deleted on chromosome ten in endometrial cancer ishikawa cells

Figure 2

MiR-205 targets PTEN at 3′-UTR. (A) Sequences of miR-205 binding sites with PTEN 3′-UTR. Red nucleotides are mutated nucleotides of PTEN 3′-UTR. (B) Schematic of the Dual-Luciferase pmiR-RIBOBIO reporter vector constructs. The 3′-UTR region of PTEN was subcloned into the Dual-Luciferase reporter vector. (C) Effect of miR-205 on the luciferase activity of Luc-PTEN 3′-UTR WT and Luc-PTEN 3′-UTR-mut. The wild type or mutant vectors were co-transfected with miR-205 mimics or negative control miRNA in Ishikawa cells. Luciferase activity was significantly decreased in miR-205+ PTEN WT group compared with miR-205+ PTEN mut (p = 0.002) or NC miRNA + PTEN WT groups (p = 0.005). Each sample was normalized to Renilla luciferase activity. (RLU, relative luciferase units: firefly luciferase/Renilla luciferase). Data are the geometric means of three independent experiments, performed in duplicates.

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