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Figure 3 | BMC Cancer

Figure 3

From: RITA (Reactivating p53 and Inducing Tumor Apoptosis) is efficient against TP53 abnormalmyeloma cells independently of the p53 pathway

Figure 3

Silencing p53 did not inhibit the myeloma cell death induced by RITA. A, D : Dose responses of shCt and shp53 TP53wt or TP53mut cells to RITA (A,B) and nutlin3a (C,D) were evaluated as described in the legend of Figure 1. For RITA, the LD50 values (μM) of shCt and shp53 cells were 0.20 ± 0.1 and 0.23 ± 0.03 for KMS12PE, 0.04 ± 0.01 and 0.06 ± 0.01 for XG5, 1.9 ± 0.8 and 2.7 ± 2 for XG6 and 1.8 ± 0.5 and of 1.5 ± 0.5 for NCI-H929, respectively. For nutlin3a, the LD50 values (μM) of shCt and shp53 cells were 3.9 ± 2.4 and >10 for NCI-H929, 3.1 ± 1.4 and >10 for XG6 and >10 for KMS12PE and XG5, respectively. *indicates a significant difference between shCt and shp53 HMCls (p < 0.05). E, F: Western blot analysis of p53, Noxa, Bax and caspase expression in shCt and shp53 XG5 cells treated overnight with 100 nM RITA (E) and in shCt and shp53 NCI-H929 cells treated overnight with 10 μM nutlin3a (F). *indicates that membrane was overexposed to assess constitutive p53 level in shp53 and shCont cells. P53 expression was decreased by 63% and 68% in untreated and treated NCI-H929 cells, respectively. Extinction level could not be calculated in XG5 because no p53 expression could be detected in shp53 XG5 cells.

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