Figure 3

OPNc-CM from OvCar-3 and PC-3 cells induces adhesion, proliferation and migration of HUVEC cells. (A) Endothelial cells were plated onto OPNc and EV-conditioned medium (CM) to evaluate cell adhesion. Cells were allowed to adhere for 2 hours and were quantified as described in the Methods section. Error bars represent the mean SD of 3 independent experiments. O.D., optical density measured at 550 nm. **p < 0.0001. (B) HUVECs were cultured with OPNc and EV-CM. Proliferation kinetics were evaluated by crystal violet staining. Error bars represent the mean SD of 3 independent experiments. *p < 0.05. O.D., measured at 550 nm. (C) and (D) OPNc-CM from OvCar-3 and PC-3 cells induces higher HUVEC cell migration rates. HUVEC cells were plated as indicated in the Methods section and analyzed for cell migration by calculating wound area, which was assessed after 6 h. Phase-contrast photomicrographs were taken at 0 and 6 h after migration was initiated. Wound margins are shown at time points 0 (upper panels) and 6 hours (lower panels). The columns show the% of mean wound area ± SD for three independent experiments. *p < 0,05.