Figure 7

Role of PKC and EGFR in LPA-induced cell migration. A: Role of PKC: Cell migration after scratch wounding in a confluent cell layer. Inhibitor, solvent (DMSO) and/or TPA (0.01 μM), LPA (10 μM) or EGF (5 nM) was added to the wells with serum-free medium as indicated. Digital images were obtained immediately after stimulation and after 24 h (E10) or 48 h (SCC-9). PKC inhibitor (GF109203X) abolished LPA-induced cell migration slightly dose-dependent (stronger inhibition with 5 μM than with 2.5 μM). TPA gave a small induction of cell migration in the E10 cells and a larger induction in the SCC-9 cells that was inhibited by GF, confirming PKC-involvement. n = 3. B: Role of downstream signalling pathways: Cell migration after scratch wounding in a confluent cell layer: Inhibitor, solvent (DMSO) and/or LPA were added to the wells with serum-free medium as indicated. Digital images were obtained immediately after stimulation and after 24 h. C: Role of EGFR: Cell migration after scratch wounding in a confluent cell layer. Inhibitors, solvent (DMSO) and/or LPA were added to the wells with serum-free medium as indicated. Digital images were obtained immediately after stimulation and after 24 h (E10), 48 h (SCC-9), and 17 h (D2) and wound closure was measured. Bars represent mean ± SEM, n = 3, 4 or 5. LPA 10 μM, gefitinib 1 μM, cetuximab 0.16 μM, GF109203X 2.5 μM and 5 μM, LY294002 10 μM, PD98059 10 μM, SB203580 10 μM. *indicates p < 0.001.