Figure 3

Effects of LPAR specific agonists. LP-105 (agonist for LPAR2), VPC31143(R) (agonist LPAR) and (2S)-OMPT (agonist for LPAR3) were used. A: Serum-starved E10 and SCC-9 cells were stimulated for 3 or 5 min with LPA, LP-105, OMPT or VPC, and blots were detected for pERK, pAkt and pEGFR. Similar phosphorylation levels were seen with LPA, VPC and OMPT in both cell lines. No phosphorylation of EGFR was detected in SCC-9 cells with any of the agonists. B: Cell migration measured in wound assays. Left panel: E10 cells were stimulated with LPAR-agonists as indicated and migration was measured at 48 h, n = 3. Bars represent mean ± SEM. *indicates p < 0.05 compared to control. VPC (2.5 μM) and OMPT (2.5 μM) gave similar results as LPA (p < 0.05), while no migration was induced by LP-105. Right panel: Dose–response curve for LPAR3 agonist OMPT in E10 cells from one representative experiment show full wound closure with 2.5 μM OMPT at 48 h.