Figure 1

Effect of LPA on migration and invasiveness in oral carcinoma cells. A: The effect of various GPCR ligands on cell migration. Cell migration was observed after scratch wounding in a confluent cell layer. Various GPCR ligands were added to the wells with serum-free medium as indicated. Digital images were obtained immediately after stimulation and after 17 h (D2), 24 h (E10) or 48 h (SCC-9) and wound closure was measured. The dark marks on the images were made to orient the picture and ensure that they were from the same location. B: Dose-dependent wound closure with LPA stimulation in the E10, SCC-9 and D2 cells. Cell migration was measured after scratch wounding in a confluent cell layer as in A. LPA was added to the wells with serum-free medium as indicated. Digital images were obtained immediately after stimulation and after 17 h (D2), 24 h (E10) or 48 h (SCC-9) and per cent wound closure was measured. Bars represent mean ± SEM (Standard error of the mean), n = 5. Left: Dose/response curve for one experiment in E10 cells is shown with doses from 0 to 100 μM, indicating a near maximal effect at 24 h using 10 μM LPA. 10 μM of LPA was chosen for use in further experiments. C: Organotypic coculture model without (left) and with (right) 10 μM LPA added during day 4 to 11 of culture.