Wee1 siRNA treatment results in mistimed cell cycle events in NCI-H1299 cells. (a) NCI-H1299 cells were synchronized in S-phase using double thymidine block and treated with non-targeting (N1) or Wee1 (#8) siRNAs. DNA content was measured by FACS analysis of PI staining of cells at 0, 4, 8 and 24 hours after release. (b) NCI-H1299 cells were treated as in (a) and directly lysed and analyzed by Western blotting at 0 and 32 hours after release. (c) NCI-H1299 cells were treated as in (a) and representative light microscopy phenotypic images were captured 24 hours after release.