Figure 6

Wee1 knockdown causes dramatic cell cycle effects cell cycle in a subset of solid tumor lines. Cells were subjected to 5nM non-targeting (N1) or Wee1 siRNAs (#6 and #8) and stained with PI for FACS analysis. NCI-H1299 cells were analyzed for DNA content at 24, 48 and 72 hours after siRNA transfection. DNA content of A549, Daoy, D54MG and A2780 cells were analyzed at 72 hours after siRNA transfection.