Wee1 knockdown results in potent inhibition of proliferation in a subset of human tumor cell lines. (a-e) NCI-H1299, A549, Daoy, D54MG and A2780 cell lines were subjected to 5 nM siRNA treatment for five days following plating and end-point analysis was performed using CellTiter-Glo . Controls included no siRNA (No), non-targeting siRNAs (NT1 and NT2) as negative controls and Ran siRNA (Ran) as a positive control for transfection efficiency. Four individual Wee1 siRNAs were used (#5-#8). Percent inhibition was calculated using the negative control (No siRNA) as 0% inhibition and the positive control (Ran siRNA) as 100% inhibition to control for relative transfection in each individual cell line. Results represent triplicate counts from a representative experiment with error bars representing s.e.m.