Verification of protein expression in the cell culture system. Western blot analysis was performed on a cell culture system. MCF-7aro cells were cultured and treated with 10 nM androstenedione and aspirin or celecoxib for 72 hr. Protein was extracted from the cells. The upper panel (A) is the image for aspirin-treated samples and celecoxib-treated samples are shown in the lower panel (B).