Protein expression of COX-2 and genes related to cell cycle and apoptosis. COX-2, cell cycle and apoptosis regulatory proteins were determined by western blot analysis, and the image is shown in A. The corresponding optical density reading is shown in B. Groups labeled as AD, AD+celecoxib, and AD+aspirin are the mice treated with androstenedione, androstenedione and celecoxib, and androstenedione and aspirin, respectively. Values are means ± SEM, n=3 to 5. The data was analyzed by One-way ANOVA, followed by Tukey’s Multiple Comparison test when P<0.05. Means labeled with different letter are significantly different (order: b>a).