The impact of the
3’UTR-variant on luciferase expression in breast cancer cell lines. Dual luciferase reporters harboring either the non-variant (G-allele, dark grey) or variant (T-allele, light grey) BRCA1 3’UTR were transiently transfected into various breast cancer cell lines (as indicated). Following a 16-hour incubation the cells were lysed and luciferase activities were analyzed. Renilla luciferase was normalized to firefly luciferase. T-allele expression was calculated relative to that of the G-allele. Plotted is the mean and standard deviation of 4 independent experiments. *p < 0.05; error bars represent the mean ± standard deviation.