BCL6 as the direct target gene of miR-339-5p in breast cancer cells.
(a) qRT-PCR and Western blot. miR-339-5p mimics or miR-339-5p ASO was transiently transfected into T47D cells and subjected to analysis of BCL6 expression. (b) The binding site of BCL6 3′-UTR and miR-339-5p. (c) Luciferase reporter assay. T47D cells were transfected with psiCHECK-2-BCL6 3′-UTR or psiCHECK-2-BCL6 mutated 3′-UTR plus either miR-339-5p mimics or negative control and subjected to luciferase reporter assay. (d) Tumor cell migration and invasion assay and Western blot. MCF-7 cells were grown and transiently transfected with miR-339-5p ASO, miR-339-5p ASO plus BCL6 siRNA or scrambled sequence oligonucleotides as negative control for 2 days and subjected to migration, invasion and western blot assays. *P < 0.05; **P < 0.01.