Effects of BCL6 expression on regulation of breast cancer colony formation and migration and invasion capacity.
(a) Soft agar assay. After gene transfection, cells were seeded in 0.35% top agarose and 10% FBS in six-well plates in triplicate. The number of colonies was counted after 14 days incubation. (b) Tumor cell migration and invasion assay. MDA-MB-453 cells were grown and transiently transfected with BCL6 siRNA or negative control (NC) for 72 h. MCF-7 cells were grown and transiently transfected with BCL6 cDNA or vector-only (VEC) for 48 h. Cells in the upper chamber were removed and those cells migrated to the lower layer of the inner chamber were stained and counted. **, P < 0.01.