Figure 3From: B-cell lymphoma 6 protein stimulates oncogenicity of human breast cancer cells Effects of BCL6 expression on regulation of breast cancer colony formation and migration and invasion capacity. (a) Soft agar assay. After gene transfection, cells were seeded in 0.35% top agarose and 10% FBS in six-well plates in triplicate. The number of colonies was counted after 14 days incubation. (b) Tumor cell migration and invasion assay. MDA-MB-453 cells were grown and transiently transfected with BCL6 siRNA or negative control (NC) for 72 h. MCF-7 cells were grown and transiently transfected with BCL6 cDNA or vector-only (VEC) for 48 h. Cells in the upper chamber were removed and those cells migrated to the lower layer of the inner chamber were stained and counted. **, P < 0.01.Back to article page