Figure 3
PGE 2 -stimulated cAMP accumulation in pancreatic stellate cells. A. Dose-dependent effect of PGE2 in the absence and presence of 0.5 mM isobutylmethylxanthine (IBMX). Cells were cultured as described in Methods, and were stimulated for 5 minutes. Results are presented as mean + S.E.M. of three replicates from one representative of three experiment. B. Effect of EP4 receptor antagonist (L161982, 10 μM) and EP2 receptor antagonist (AH6809, 30 μM) on PGE2-stimulated cAMP accumulation (1 μM PGE2). Cells were preincubated with antagonists for 30 minutes before stimulation with PGE2 for 15 minutes in the presence of 0.5 mM IBMX. Results are presented as mean ± S.E.M. of five experiments.