Figure 2

Induction of COX-2 protein in pancreatic stellate cells by indirect coculture with pancreatic adenocarcinoma cell lines. A. Effect of coculture with Panc-1, HPAFII, and BxPC-3 cells. Cells were cocultured in serum-free medium for 48 hours, before harvesting and analysis by Western blots as described in Methods. Results are from one typical of three experiments. B. Inhibition of COX-2 induction by coculture with BxPC-3 cells when stellate cells were pretreated with IL-1 receptor antagonist (1 μg/ml) for 1 hour before coculture for 48 hours. Results are from one typical of four experiments.