The effects of HA-1077 (0–30 μM) on migration in 5637 cell. The cancer cells were incubated in supplemented with HA-1077 with/without LPA and GGOH. (A) The percentage of migrated cells decreased in dose-dependent manner. LPA and GGOH blocked HA-1077 induced suppression of migration. (B-D) RhoA activity was not statistically different between with and without HA-1077, but ROCK-I and ROCK-II expression was significantly reduced in the cells on the underside of each filter. The data show the 95% confidential interval.