Figure 5

Inhibiting effect of HA-1077 on proliferation and RhoA activity and ROCK expression in UM-UC-3 cell using the 3-(4,5-dimethyl-2-thiazolyl)-2,5diphenyl-2H-tetrazolium (MTT) assay. The ratio of the cells treated with various doses (0.5–30 μM) of HA-1077 to control cells (HA-1077 0 μM) set as 1.0 were calculated. HA-1077 inhibited the growth of UM-UC-3 cell in a dose-dependent manner (A). HA-1077 did not decrease the RhoA activity (B), but inhibited ROCK-I (C) and ROCK-II (D) in dose-dependent manner. The data show the 95% confidential interval.