Figure 3From: COUP-TFI modifies CXCL12 and CXCR4 expression by activating EGF signaling and stimulates breast cancer cell migration Estrogenic regulation of CXCL12 and CXCR4 in control and COUP clones. Control (Cont.) and COUP clones were treated with ethanol (EtOH) as the vehicle or E2 10−8 M and ICI 10−6 M alone or both together for 48 h. The CXCL12 (A) and CXCR4 (B) relative mRNA levels were monitored by a real-time PCR analysis, normalized to GAPDH mRNA as the internal control, and were expressed as the relative mRNA expression of CXCL12 or CXCR4. Data are the mean ± SEM of at least three independent experiments. The asterisks indicate significant differences (p < 0.05) between the untreated and treated control clones. The pound sign indicates significant differences (p < 0.05) between the untreated and treated COUP clones.Back to article page