Figure 5
From: Lipid metabolism enzyme ACSVL3 supports glioblastoma stem cell maintenance and tumorigenicity
Activation of receptor tyrosine kinase (RTK) signaling pathways induced ACSVL3 expression in GBM stem cell enriched neurospheres. A. Incubation with EGF (30 ng/ml) or HGF (20 ng/ml) for 48 hours induced ACSVL3 expression in HSR-GBM1A, HSR-GBM1B and two primary low passage neurosphere cultures from GBM patients (JHH612, JHH626). B. Cells were pre-incubated with a small-molecule inhibitor of c-Met, SU11274 (20 μmol/L) for 6 hours and then treated with HGF (20 ng/mL) for 48 hour prior to immunoblotting analysis. Inhibition of the HGF/c-Met pathway reversed ACSVL3 induction by HGF.