Figure 5

miR-26b downregulates USP9X via directly targeting the USP9X 3′UTR. (A) Representative images of cells in the lower section of a transwell chamber are shown to demonstrate the invasive properties of Huh7 and Hep3B cells when transfected with a mixed miR-26b and USP9X vector, or a mixed miR-26b and USP9X-3′UTR (left panel). Quantitative analyses of migrated cells are also shown ( right panel); data are plotted as the average number of cells per field of view from three different experiments (original magnification: ×400). (B) The relative expression level of miR-26b is shown for five normal liver tissue samples (N), and nine HCC liver tissue samples (upper right panel). Below, the corresponding expression levels of USP9X, E-cadherin and β-actin are shown in the same samples. Linear regression analyses of miR-26b expression with USP9X and E-cadherin are also presented (right panel).