Figure 3

Increased levels of miR-26b suppress EMT in HCC cell lines. (A) The invasive properties of HCC cells treated with the empty vector, miR-26b mimic and miR-26b inhibitor were analyzed using a cell invasion assay in transwell chambers. Representatives images of cells that had migrated into the lower chamber are shown (left panel; original magnification: ×400), and quantitative data are also presented (right panel). The average number of cells per field of view from three different experiments are plotted. (B) The invasive properties of HCC cells treated with empty vector, miR-26b mimic or miR-26b inhibitor were also performed using a 3D spheroid invasion assay. Representative images of cells after 8 days in culture are shown (upper; original magnification:: ×200, bottom; original magnification:: ×400).