Figure 2

Increased levels of miR-26b suppress mobolity and E-cadherin expression in HCC cell lines. (A) Real-time PCR analysis of expression of miR-26b in indicated cells. U6 was used as a loading control. Error bars represent mean ± SD from three independent experiments. (B) Western blotting analysis of USP9X, SMAD4, E-cadherin and vimentin expression in indicated cells. (C) Real-time PCR analysis of expression of E-cadherin and vimentin in indicated cells. GAPDH was used as a loading control. Error bars represent mean ± SD from three independent experiments. (D) A wound closure assay was used to assess the mobility of Huh7 and Hep3B cells treated with the empty vector, miR-26b mimic or miR-26 inhibition (original magnification: ×20;).